Limit of detection:
Food crops (wheat, barley, corn, soybeans, soybeans, sorghum, peanuts, oats, etc.), feed raw materials (bran, cottonseed, rapeseed meal, rice bran, distiller's grains, DDGS, sprayed corn bran, etc.) , Finished feed (livestock and poultry feed): 3-50ppb (ng/g)
Peanut meal: 10-100ppb (ng/g)
Silage, cotton seed straw and other water-absorbing samples: 50ppb (ng/g)
Components
1. Rapid test strip, 96Test/ box
2. Gold microwell, 96 wells/ box
3. Sample diluent
4. Instruction
Store at 20±5°C.
Shelf time is 12 months.
Equipment and reagents to be used but not provided
1 Micropipette
2 Electronic balance
3 Vortex instrument
4 Centrifuge
5. 70% methanol-water solution: Accurately measure 70mL methanol (analytical grade) and 30mL deionized water, mix well and set aside .
Sample Preparation:
Samples 1 Food crops, feed ingredients, finished feed
1.Grind the sample into powder;
2. Weigh 1.00±0.05g homogenized sample into a 10mL centrifuge tube;
3. Detection limit 3ppb: add 70% methanol-water solution 3mL;
detection limit 5-100ppb: add 70% methanol-water solution 4mL;vortex for 5min, or wait for stratification;
4. Pipette supernatant into different sample diluent according to LOD, mix well for next step.
| Limit of Detection(ppb) | 3 | 5 | 10 | 20 | 30 | 40 | 50 |
| supernatant(μl) | 100 | 50 | 30 | 30 | 20 | 20 | 20 |
| Sample diluent(μl) | 300 | 500 | 600 | 770 | 950 | 1200 | 1350 |
| 70% methanol-water solution(μl) | 0 | 20 | 80 | 180 | 200 | 350 | 450 |
Samples 2 peanut meal
3. add 70% methanol-water solution 4mL;, vortex for 5min, or wait for stratification;
| Limit of Detection | 10 | 20 | 50 | 100 |
| supernatant(μl) | 10 | 10 | 10 | 10 |
| Sample diluent(μl) | 600 | 1300 | 1800 | 1800 |
| 80% ethanol-water solution(μl) | 0 | 0 | 50 | 200 |
Samples 3 Hygroscopic samples such as silage, cottonseed straw, etc.
3. Add 6mL 70% methanol-water solution, vortex for 5min, or wait for stratification;
| Limit of Detection | 50ppb |
| supernatant(μl) | 30 |
| Sample diluent(μl) | 1970 |
| 80% ethanol-water solution(μl) | 400 |
Test Progress
1. Pipette 200 μL of the sample to be tested into the gold microwell, carefully pipette until the purple particles are completely dissolved (note: be gentle when pipetting to avoid air bubbles), and incubate at room temperature for 3 minutes;
2 Insert the test strip into the gold microwell. fully immerse the sample pad in the sample and react for 5 minutes;
3 Take out the test strip from the microwell, discard the sample pad, and interpret the result within 1 minute.
Negative positive Invalid
Negative: C ≥T
Positive: C < T
Invalid: C Line does not show up
