Limit of detection:
Corn, corn gluten meal, flaked corn, lactating dairy cow feed, calf feed: 700-1000ppb (μg/kg)
sprayed corn husk, non-lactation feed, soybean meal, soybean cake, cotton meal, bran, DDGS: 3000 -5000ppb (μg/kg)
silage: 500-5000ppb (μg/kg)
Components
1. Rapid test strip, 96Test/ box
2. Gold microwell, 96 wells/ box
3. Sample diluent
4. Instruction
Store at 20±5°C.
Shelf time is 12 months.
Equipment and reagents to be used but not provided
1 Micropipette
2 Electronic balance
3 Vortex instrument
4 Centrifuge
5. 50% ethanol-water solution: Accurately measure 50mL ethanol (analytical grade) and 50mL deionized water, mix well and set aside .
Sample Preparation:
SAMPLES 1. Corn, corn gluten meal, flaked corn, lactating dairy cow feed, calf feed
1.1 Grind the samples into powder;
1.2 Weigh 1.00±0.05g into a 10mL centrifuge tube;
1.3 Add 6 mL of 50% ethanol-water solution, vortex for 3 minutes, and centrifuge at 4000 rpm for 5 minutes;
1.4 Take supernatant and sample diluent according to the following detection limit, mix well for testing.
| Limit of Detection | 700ppb | 1000ppb |
| supernatant (μL) | 180 | 180 |
| Sample diluent (μL) | 50 | 300 |
SAMPLES 2 Sprayed corn bran, non-lactation feed, soybean meal, soybean cake, cotton meal, bran
| Limit of Detection | 3000ppb | 4000ppb | 5000ppb |
| supernatant (μL) | 50 | 50 | 50 |
| Sample diluent (mL) | 0.9 | 1.3 | 1.7 |
SAMPLES 3 DDGS
| Limit of Detection | 3000ppb | 4000ppb | 5000ppb |
| supernatant (μL) | 50 | 50 | 50 |
| Sample diluent (mL) | 1.2 | 1.6 | 2 |
NOTE: The supernatant of DDGS and some finished feeds is acidic or alkaline after extraction. When the acidic pH<5 or="" alkaline="" ph="">9, it is best to use 1.0mol/L NaOH solution or 1.0mol/L HCL solution to adjust After the supernatant pH=6.0~8.0, dilute and test;
SAMPLES 4 Silage
500ppb add 4mL 50% ethanol-water solution, others add 6mL
| Limit of Detection | 500 | 100 | 3000ppb | 4000ppb | 5000ppb |
| supernatant (μL) | 100 | 50 | 50 | 50 | 50 |
| Sample diluent (mL) | 0.2 | 0.3 | 0.9 | 1.3 | 1.7 |
Test Progress
1. Pipette 200 μL of the sample to be tested into the gold microwell, carefully pipette until the purple particles are completely dissolved (note: be gentle when pipetting to avoid air bubbles), and incubate at room temperature for 3 minutes;
2 Insert the test strip into the gold microwell. fully immerse the sample pad in the sample and react for 5 minutes;
3 Take out the test strip from the microwell, discard the sample pad, and interpret the result within 1 minute.
Negative positive Invalid
Negative: C ≥T
Positive: C < T
Invalid: C Line does not show up
