Limit of detection:
2-5ppb (μg/kg) for following samples
Food crops: wheat, barley, corn, soybeans, soybeans, sorghum, peanuts, oats, etc.;
feed materials: various food crops bran, soybean meal, rice bran, distiller's grains, DDGS, sprayed corn bran, glutamic acid slag, etc.;
Finished feed: livestock and poultry feed (including silage)
3-5ppb (μg/kg) for following samples
Cottonseed, Molasses, Cotton Meal, Sesame Meal
Components
1. Rapid test strip, 96Test/ box
2. Gold microwell, 96 wells/ box
3. Sample diluent
4. Instruction
Store at 20±5°C.
Shelf time is 12 months.
Equipment and reagents to be used but not provided
1 Micropipette
2 Electronic balance
3 Vortex instrument
4 Centrifuge
5. 80% ethanol-water solution: Accurately measure 80mL ethanol (analytical grade) and 20mL deionized water, mix well and set aside .
Sample Preparation:
Samples 1 Wheat, barley, corn, soybeans, soybeans, sorghum, peanuts, oats and other food crops; finished feed; feed materials such as soybean meal
1.Grind the sample into powder;
2. Weigh 1.00±0.05g homogenized sample into a 10mL centrifuge tube;
3. Add 3mL 80% ethanol-water solution, vortex for 5min, or wait for stratification;
4. Pipette 200μl supernatant into different sample diluent according to LOD, mix well for next step.
| Limit of Detection | 2ppb | 3ppb | 5ppb |
| supernatant(μl) | 200 | 200 | 200 |
| Sample diluent(μl) | 400 | 350 | 600 |
| 80% ethanol-water solution(μl) | 0 | 0 | 90 |
Samples 2 Rice bran, distiller's grains, DDGS, sprayed corn husk and other acidic feed
3. Add 3mL 80% ethanol-water solution, vortex for 5min, or wait for stratification;
| Limit of Detection | 2ppb | 3ppb | 5ppb |
| supernatant(μl) | 200 | 200 | 200 |
| Sample diluent(μl) | 450 | 400 | 600 |
| 80% ethanol-water solution(μl) | 0 | 0 | 90 |
NOTE: The supernatant of DDGS and some finished feeds is acidic or alkaline after extraction. When the acidic pH<5 or alkaline pH>9, it is best to use 1.0mol/L NaOH solution or 1.0mol/L HCL solution to adjust After the supernatant pH=6.0~8.0, dilute and test;
Samples 3 Bran and other highly absorbent samples and silage
3. Add 5mL 80% ethanol-water solution, vortex for 5min, or wait for stratification;
| Limit of Detection | 2ppb | 3ppb | 5ppb |
| supernatant(μl) | 200 | 200 | 200 |
| Sample diluent(μl) | 500 | 400 | 600 |
| 80% ethanol-water solution(μl) | 0 | 0 | 90 |
Samples 4 Cottonseed, molasses, cottonseed meal, sesame meal, glutamic acid slag
3. Add 5mL 80% ethanol-water solution, vortex for 5min, or wait for stratification;
| Limit of Detection | 3ppb | 5ppb |
| supernatant(μl) | 200 | 100 |
| Sample diluent(μl) | 800 | 700 |
| 80% ethanol-water solution(μl) | 0 | 50 |
Test Progress
1. Pipette 200 μL of the sample to be tested into the gold microwell, carefully pipette until the purple particles are completely dissolved (note: be gentle when pipetting to avoid air bubbles), and incubate at room temperature for 3 minutes;
2 Insert the test strip into the gold microwell. fully immerse the sample pad in the sample and react for 5 minutes;
3 Take out the test strip from the microwell, discard the sample pad, and interpret the result within 1 minute.
Negative positive Invalid
Negative: C ≥T
Positive: C < T
Invalid: C Line does not show up
