Mycotoxin ZEN Zearalenone Rapid Test strip for grain and feed 60-1000ppb

Limit of detection:

60-1000ppb (μg/kg) for following samples

Food crops (wheat, barley, corn, soybeans, soybeans, sorghum, peanuts, oats, etc.), feed materials (rapeseed meal, rice bran, distiller's grains, DDGS, sprayed corn husks, etc.), finished feed (livestock and poultry feed)

Bran: 100ppb (μg/kg)

Components

1. Rapid test strip, 96Test/ box 

2. Gold microwell, 96 wells/ box

3. Sample diluent (10X)

4. Instruction

Store at 20±5°C.

Shelf time is 12 months.

Equipment and reagents to be used but not provided

1 Micropipette

2 Electronic balance

3 Vortex instrument

4 Centrifuge

5. 70% Methanol-water solution: Accurately measure 70mL Methanol and 30mL deionized water, mix well and set aside .

6. Sample diluent: Mix the diluted sample diluent (10×) with deionized water at a ratio of 1:9 (1 part of diluted sample diluent (10×) + 9 parts of deionized water), set aside.

 Sample Preparation: 

SAMPLES 1: Acidic feed such as rice bran, distiller's grains, DDGS, sprayed corn husk and others

1.1 Grind the samples into powder;

1.2 Weigh 1.00±0.05g into a 10mL centrifuge tube;

1.3 Add 4mL of 70% Methanol-water solution, vortex for 3 minutes, and centrifuge at 4000 rpm for 5 minutes;

1.4 Take 20 μL of supernatant and add it to the sample diluent according to the following detection limit, Vortex 10 seconds.

SAMPLES 2 bran

1.4 Take 40 μL of supernatant and add it to the sample diluent according to the following detection limit, Vortex 10 seconds.

Test Progress

1. Pipette 200 μL of the sample to be tested into the gold microwell, carefully pipette until the purple particles are completely dissolved (note: be gentle when pipetting to avoid air bubbles), and incubate at room temperature for 3 minutes;

2 Insert the test strip into the gold microwell. fully immerse the sample pad in the sample and react for 5 minutes;

3 Take out the test strip from the microwell, discard the sample pad, and interpret the result within 1 minute.

             Negative       positive         Invalid

Negative: C ≥T 

Positive: C < T 

Invalid: C Line does not show up

NOTE: The supernatant of DDGS and some finished feeds is acidic or alkaline after extraction. When the acidic pH<5 or="" alkaline="" ph="">9, it is best to use 1.0mol/L NaOH solution or 1.0mol/L HCL solution to adjust After the supernatant pH=6.0~8.0, dilute and test;