Limit of detection:
100-800ppb (μg/kg) for following samples
Food crops: wheat, barley, corn, soybeans, soybeans, sorghum, peanuts, oats, etc.;
Feed raw materials: various grain crop bran, cottonseed, rapeseed meal, soybean meal, rice bran, distiller's grains, DDGS, sprayed corn bran, corn gluten meal, etc.
Finished feed: livestock and poultry feed (including silage);
Components
1. Rapid test strip, 96Test/ box
2. Gold microwell, 96 wells/ box
3. Sample diluent (10X)
4. Instruction
Store at 20±5°C.
Shelf time is 12 months.
Equipment and reagents to be used but not provided
1 Micropipette
2 Electronic balance
3 Vortex instrument
4 Centrifuge
5. 70% Methanol-water solution: Accurately measure 70mL methanol and 30mL deionized water, mix well and set aside .
6. Sample diluent: Mix the diluted sample diluent (10×) with deionized water at a ratio of 1:9 (1 part of diluted sample diluent (10×) + 9 parts of deionized water), set aside.
Sample Preparation:
SAMPLES 1:Acidic feed such as rice bran, distiller's grains, DDGS, sprayed corn husks, corn gluten powder, etc.
1.1 Grind the samples into powder;
1.2 Weigh 1.00±0.05g into a 10mL centrifuge tube;
1.3 Add 2.5 mL of 70% Methanol-water solution, vortex for 3 minutes, and centrifuge at 4000 rpm for 5 minutes;
1.4 Take 20 μL of supernatant and add it to the sample diluent according to the following detection limit, mix well for testing.
Limit of Detection | 200ppb | 400ppb | 800ppb |
Sample diluent | 2mL | 4mL | 7.5mL |
SAMPLES 2 Cottonseed, bran and other samples with strong water absorption
1.3 Add 6 mL of 70% Methanol-water solution, vortex for 3 minutes, and centrifuge at 4000 rpm for 5 minutes;
1.4 Take 100 μL of supernatant and add it to the sample diluent according to the following detection limit, mix well for testing.
Limit of Detection | 200ppb | 400ppb | 800ppb |
Sample diluent | 1mL | 2.5mL | 5.5mL |
SAMPLES 3 Silage
1.3 Add 6 mL of 70% Methanol-water solution, vortex for 3 minutes, and centrifuge at 4000 rpm for 5 minutes;
1.4 Take 20 μL of supernatant and add it to the sample diluent according to the following detection limit, mix well for testing.
Limit of Detection | 100PPB | 200ppb | 400ppb | 800ppb |
Sample diluent | 1mL | 2mL | 3mL | 7mL |
SAMPLES 4 Corn and other grain crops, livestock and poultry feed, soybean meal and other feed materials
1.3 Add 4mL of 70% Methanol ethanol-water solution, vortex for 3 minutes, and centrifuge at 4000 rpm for 5 minutes;
1.4 Take 20 μL of supernatant and add it to the sample diluent according to the following detection limit, mix well for testing.
Limit of Detection | 200ppb | 400ppb | 800ppb |
Sample diluent | 2mL | 4mL | 8mL |
Test Progress
1. Pipette 200 μL of the sample to be tested into the gold microwell, carefully pipette until the purple particles are completely dissolved (note: be gentle when pipetting to avoid air bubbles), and incubate at room temperature for 3 minutes;
2 Insert the test strip into the gold microwell. fully immerse the sample pad in the sample and react for 5 minutes;
3 Take out the test strip from the microwell, discard the sample pad, and interpret the result within 1 minute.
Negative positive Invalid
Negative: C ≥T
Positive: C < T
Invalid: C Line does not show up
NOTE: The supernatant of DDGS and some finished feeds is acidic or alkaline after extraction. When the acidic pH<5 or="" alkaline="" ph="">9, it is best to use 1.0mol/L NaOH solution or 1.0mol/L HCL solution to adjust After the supernatant pH=6.0~8.0, dilute and test;