Limit of detection:
50-100ppb (μg/kg) for following samples
Food crops: wheat, barley, corn, soybeans, soybeans, sorghum, peanuts, oats, etc.;
Feed materials: various food crops bran, cotton meal, rapeseed meal, rice bran, distiller's grains, DDGS, sprayed corn bran, etc.;
Finished feed: livestock and poultry feed (including silage);
Components
1. Rapid test strip, 96Test/ box
2. Gold microwell, 96 wells/ box
3. Sample diluent
4. Instruction
Store at 20±5°C.
Shelf time is 12 months.
Equipment and reagents to be used but not provided
1 Micropipette
2 Electronic balance
3 Vortex instrument
4 Centrifuge
5. 50% ethanol-water solution: Accurately measure 50mL ethanol (analytical grade) and 50mL deionized water, mix well and set aside .
Sample Preparation:
1.Grind the sample into powder;
2. Weigh 1.00±0.05g homogenized sample into a 10mL centrifuge tube;
3. Add 4mL 50% ethanol-water solution, vortex for 5min, or wait for stratification;
4. Pipette 100μl supernatant into different sample diluent according to LOD, mix well for next step.
| Limit of Detection | 50ppb | 100ppb |
| Sample diluent | 400μl | 700μl |
Test Progress
1. Pipette 200 μL of the sample to be tested into the gold microwell, carefully pipette until the purple particles are completely dissolved (note: be gentle when pipetting to avoid air bubbles), and incubate at room temperature for 3 minutes;
2 Insert the test strip into the gold microwell. fully immerse the sample pad in the sample and react for 5 minutes;
3 Take out the test strip from the microwell, discard the sample pad, and interpret the result within 1 minute.
Negative positive Invalid
Negative: C ≥T
Positive: C < T
Invalid: C Line does not show up
