Mycotoxins T-2 Toxin 100ppb-200ppb-500ppb Rapid Test Strip for grain and feed

Limit of detection:

200ppb-500ppb (μg/kg) for following samples

Food crops: wheat, barley, corn, soybeans, soybeans, sorghum, peanuts, oats, etc.;

Feed materials: various food crops bran, cotton meal, rapeseed meal, rice bran, distiller's grains, sprayed corn bran, etc.;

Finished feed: livestock and poultry feed (including silage); 

100ppb-200ppb (μg/kg) for DDGS

Components

1. Rapid test strip, 96Test/ box 

2. Gold microwell, 96 wells/ box

3. Sample diluent

4. Instruction

Store at 20±5°C.

Shelf time is 12 months.

Equipment and reagents to be used but not provided

1 Micropipette

2 Electronic balance

3 Vortex instrument

4 Centrifuge

5. 50% ethanol-water solution: Accurately measure 50mL ethanol (analytical grade) and 50mL deionized water, mix well and set aside .

 Sample Preparation: 

1. Sample :Wheat, barley, corn, soybeans, soybeans, sorghum, peanuts, oats and other food crops; finished feed; soybean meal and other feed materials; rice bran, distiller's grains, sprayed corn husks and other acidic feed

1.1 Grind the sample into powder ;

1.2 Weigh 1.00±0.05g homogenized sample into a 10mL centrifuge tube;

1.3 Add 4mL of 50% ethanol-water solution;

1.4 After vigorously shaking for 5min, let it stand for stratification or centrifuge at 4000g for 5min;

1.5 According to the required detection limit, dilute with the liquid volume shown in the table below, mix well and set aside

 2. Sample :Cotton meal, bran and other highly absorbent samples and silage

2.1 Grind the sample into powder;

2.2 Weigh 1.00±0.05g of the homogenized sample into a 10mL centrifuge tube;

2.3 Add 6mL of 50% ethanol-water solution;

2.4 After shaking vigorously for 5min, let stand for stratification or centrifuge at 4000g for 5min;

2.5 According to the required detection limit, dilute with the liquid volume shown in the table below, mix well and set aside

 3. DDGS-100PPB

3.3 Add 50% ethanol-water solution 2.5mL

3.5 Add 100 μL of the supernatant  to 500 μL of the sample diluent, mix well and set aside.

4. DDGS-200PPB

4.3 Add 50% ethanol-water solution 4mL

4.5 Add 150 μL of the supernatant  to 500 μL of the sample diluent, mix well and set aside.

 Test Progress

1. Pipette 200 μL of the sample to be tested into the gold microwell, carefully pipette until the purple particles are completely dissolved (note: be gentle when pipetting to avoid air bubbles), and incubate at room temperature for 3 minutes;

2 Insert the test strip into the gold microwell. fully immerse the sample pad in the sample and react for 5 minutes;

3 Take out the test strip from the microwell, discard the sample pad, and interpret the result within 1 minute.

              Negative      positive        Invalid

Negative: C ≥T 

Positive: C < T 

Invalid: C Line does not show up