Ethoxyquin ELISA Kit

【Product Description】 

The kit (Art. No.: KITW690.01) is an enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of Ethoxyquin in tissue of shrimp, besides the specificity of the kit has been evaluated by analyzing the cross-reactivities to corresponding substances. The kit is a convenient, fast, and sensitive assay for on-site large-scale screening of Ethoxyquin in shrimp.

【Technical Specifications】

1. Sensitivity: 0.5 ppb.

2. Detection Limit: 5ppb, shrimp

3. Recovery rate: 80%-110%, shrimp 

4. Specificity: Ethoxyquin-------------------------------------- 100% 

5. Precision： CV < 10%

【Storage instructions】 

1. Store the kit/reagents at 2-8℃ (35.6-46.4℉). The shelf life is 12 months when properly stored. 

2. Return unused micro-wells to their original foil bag and reseal them together with the desiccant provided and further store at 2-8℃ (35.6-46.4℉).

【Materials Required but not Provided】 

1. Equipment: 

a. Microtiter plate reader (450 nm)

b. Precision balance (0.01 g) 

c. Vortex 

d. Centrifuge (4000g) 

e. Graduated micropipette/ tips f. Timer

2. Reagent: 

a. Acetonitrile – AR 

b. MgSO4– AR c. Distilled water 

【Reagent Preparation】 

1. Wash Solution: Mix 1 volume of Wash Solution Concentrate (20×) with 19 volumes of distilled water. 

2. Sample diluent: Mix 1 volume of Sample diluent concentrate (4×) with 3 volumes of distilled water. 

3. Standards Solution: Mix 1 volume of each Standards concentrate (20×) with 19 volumes of Standards diluent.