Nitrofurantoin metabolites ELISA kit

【Technical Specifications】
1. Sensitivity: 0.01ng/g or ppb.
2. Detection Limit: 0.1 ng/g or ppb;Fish,Shrimp,Chicken,Duck,Pork.
3. Recovery rate: 60%-120%.
4. Specificity:
AHD---------------------------------------100%
AMOZ-----------------------------------<0.1%
SEM--------------------------------------<0.1%
AOZ--------------------------------------<0.1%
5. Precision： CV < 10%

【Test Procedure】
1. Insert appropriate number of strips into a micro well holder. Record standard and sample
positions. It is suggested that all standards and samples be run in duplicate.
2. Add 50 μL of each standard or sample (prepared according to the manual served with
Combination Ⅱ Reagents Kit) in duplicate into different wells.
3. Add 50 μL of Enzyme Conjugate Solution to each well. Then add 50 μL of Antibody Solution
as well.
4. Cover it with Adhesive Plate Sealer, and mix gently by rocking the plate for 10 s. Incubate for
30 min at room temperature(25±2℃).
5. Open the Adhesive Plate Sealer and dump the liquid in wells.
6. Wash the plate 4 times with 260 μL of Wash Solution.
7. After the last wash, tap dry the plate on paper towels.
8. Add 100 μL of TMB Substrate solution into each well.
9. Repeat step 4 but incubate for 15-20 min at room temperature.
10. Open the Adhesive Plate Sealer and add 50 μL of Stop Solution into each well. Mix gently by
rocking for 10 s.
11. Read the plate with a microtiter plate reader using a 450 nm filter within 5 min.(Air bubbles
should be eliminated prior to reading plate as they may affect analytical result.)