【Production Description】
The kit consists of enzyme mix，fluorescence PCR mix，positive control and negative control for real-time RT-PCR amplification of PRRSV.
【Test Procedure】
1. Sample preparation
Follow the commercial RNA extraction kit manual for the RNA extraction.
2. PCR
(1) Take out the reagents in the room temperature for melting, mixing and vortex.
Centrifuge briefly.
(2) Prepare the RT-PCR master mix ： combine the following components for the
number of reactions required plus 10% overage：
